FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l05n01_lib_e10.351000000a230c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l05n01_lib_e10.351000000a230c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15697599
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT448310.2855914461823111No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA196180.1249745263590948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG46750.014.68282146-47
GTATGCC50600.013.424787544-45
CGTATGC51800.013.15964144-45
GAGCACA119050.012.4877149
GCACACG59050.012.26642210-11
AGAGCAC116750.012.245538
TAGCTTA56500.011.85384834-35
CGGGGAT42500.011.8514941
CGGGAAT44450.011.7591811
CTCGTAT60400.011.44277642-43
ATGCCGT60500.011.38507646-47
ACGTCTG62500.011.36136414-15
ATCGGAA121150.011.2152462
TCGTATG61700.011.08619942-43
CCCGTAT13500.010.911491
ACACGTC66750.010.81584112-13
CCCGCAT24700.010.7732621
TCGGAAG128350.010.7711473
CACACGT66750.010.74468412-13
CGCGTAT15600.010.356441
Produced by FastQC (version 0.11.2)