FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l05n01_lib_e1.351000000a1ea5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l05n01_lib_e1.351000000a1ea5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18708359
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC58450.012.71914844-45
TATGCCG58150.012.66214646-47
GTATGCC62000.011.64608944-45
CCCGCAT28650.011.4435011
CGGGGAT49950.011.3199861
GAGCACA153800.010.2528999
TCGTATG72300.010.24932842-43
CTCGTAT72000.010.22605842-43
ATGCCGT76150.010.10579346-47
CTCGGAT95100.09.8927971
CTCGAAT97900.09.6583921
AGAGCAC162200.09.6047928
CGGGCAT28800.09.2391071
ACGTCTG79800.09.22555714-15
CGGAAAT46600.08.9728661
CGCGAAT38300.08.9324011
CTGGAAT123950.08.8935651
GCACACG86200.08.87121310-11
CGGGAAT56450.08.8381181
ACACGTC84800.08.82162312-13
Produced by FastQC (version 0.11.2)