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        Note that additional data was saved in multiqc_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2017-10-24, 21:10 based on data in: /mnt/gencore/sites/core-fastqc.bio.nyu.edu/html/C8R14ACXX/4

        Welcome! Not sure where to start?   Watch a tutorial video   (6:06)

        General Statistics

        Showing 12/12 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        C8R14ACXX_l04n01_lib_d1.351000000a1dcf
        42.0%
        46%
        8.6
        C8R14ACXX_l04n01_lib_d10.351000000a21bb
        56.2%
        41%
        7.5
        C8R14ACXX_l04n01_lib_d11.351000000a2234
        49.8%
        45%
        12.0
        C8R14ACXX_l04n01_lib_d12.351000000a2284
        50.2%
        47%
        20.7
        C8R14ACXX_l04n01_lib_d2.351000000a1e11
        37.2%
        46%
        3.6
        C8R14ACXX_l04n01_lib_d3.351000000a1e7e
        47.6%
        45%
        11.2
        C8R14ACXX_l04n01_lib_d4.351000000a1ef5
        46.1%
        46%
        10.7
        C8R14ACXX_l04n01_lib_d5.351000000a1f47
        54.5%
        46%
        17.8
        C8R14ACXX_l04n01_lib_d6.351000000a1fcd
        47.0%
        46%
        12.3
        C8R14ACXX_l04n01_lib_d7.351000000a2043
        47.1%
        46%
        10.1
        C8R14ACXX_l04n01_lib_d8.351000000a20c9
        47.4%
        46%
        10.6
        C8R14ACXX_l04n01_lib_d9.351000000a2135
        49.0%
        47%
        11.6

        Lane Statistics

        Lane Statistics

        Showing 1/1 rows and 4/4 columns.
        LaneTotal # of Single-End ReadsTotal # PF ReadsUndetermined % PhiX Aligned
        4.0
        143861314
        140913883
        3.0
        2.53%

        Lane 4 Demultiplexing Report

        Lane 4 Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.
        Perfect: The percentage of reads in this library which have a barcode perfectly matching the barcode specified in TuboWeb. The remainder of reads have mismatches upto the max number of mismatches specified in TuboWeb (Allowed barcode mismatch option in your Library Pool).

        Showing 12/12 rows and 3/3 columns.
        LibraryTotal Read CountPortion (%)Perfect (%)
        lib_d11
        11953363
        8.7
        98.8
        lib_d1
        8617183
        6.3
        99.3
        lib_d3
        11248625
        8.2
        99.4
        lib_d4
        10672273
        7.8
        99.1
        lib_d9
        11606260
        8.5
        99.2
        lib_d8
        10553608
        7.7
        99.3
        lib_d7
        10145187
        7.4
        99.2
        lib_d6
        12334324
        9.0
        98.9
        lib_d2
        3564319
        2.6
        99.0
        lib_d10
        7470909
        5.5
        99.3
        lib_d5
        17809912
        13.0
        99.5
        lib_d12
        20736088
        15.2
        99.5

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms
        12
        0
        0

        The mean quality value across each base position in the read. See the FastQC help.

        Created with Highcharts 5.0.6Position (bp)Phred ScoreChart context menuExport PlotMean Quality Scores102030405060708090100051015202530354045Created with MultiQC

        Per Sequence Quality Scores
        12
        0
        0

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

        Created with Highcharts 5.0.6Mean Sequence Quality (Phred Score)CountChart context menuExport PlotPer Sequence Quality Scores0510152025303502M4M6M8M10MCreated with MultiQC

        Per Base Sequence Content
        0
        0
        12

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content
        0
        5
        7

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

        Created with Highcharts 5.0.6%GCCountChart context menuExport PlotPer Sequence GC Content010203040506070809010001234567Created with MultiQC

        Per Base N Content
        12
        0
        0

        The percentage of base calls at each position for which an N was called. See the FastQC help.

        Created with Highcharts 5.0.6Position in Read (bp)Percentage N-CountChart context menuExport PlotPer Base N Content01020304050607080901000123456Created with MultiQC

        Sequence Length Distribution
        12
        0
        0

        All samples have sequences of a single length (101bp).

        Sequence Duplication Levels
        0
        9
        3

        The relative level of duplication found for every sequence. See the FastQC help.

        Created with Highcharts 5.0.6Sequence Duplication Level% of LibraryChart context menuExport PlotSequence Duplication Levels123456789>10>50>100>500>1k>5k>10k+020406080100Created with MultiQC

        Overrepresented sequences
        0
        9
        3

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        Created with Highcharts 5.0.6Percentage of Total SequencesChart context menuExport PlotOverrepresented sequencesTop over-represented sequenceSum of remaining over-represented sequencesC8R14ACXX_l04n01_lib_d1.351000000a1dcfC8R14ACXX_l04n01_lib_d10.351000000a21bbC8R14ACXX_l04n01_lib_d11.351000000a2234C8R14ACXX_l04n01_lib_d12.351000000a2284C8R14ACXX_l04n01_lib_d2.351000000a1e11C8R14ACXX_l04n01_lib_d3.351000000a1e7eC8R14ACXX_l04n01_lib_d4.351000000a1ef5C8R14ACXX_l04n01_lib_d5.351000000a1f47C8R14ACXX_l04n01_lib_d6.351000000a1fcdC8R14ACXX_l04n01_lib_d7.351000000a2043C8R14ACXX_l04n01_lib_d8.351000000a20c9C8R14ACXX_l04n01_lib_d9.351000000a21350%10%20%2.5%5%7.5%12.5%15%17.5%22.5%Created with MultiQC

        Adapter Content
        12
        0
        0

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

        Created with Highcharts 5.0.6Position% of SequencesChart context menuExport PlotAdapter Content10203040506070800123456Created with MultiQC

        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.