FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l04n01_lib_d9.351000000a2135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l04n01_lib_d9.351000000a2135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11606260
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600740.5175999848357696No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA298680.2573438816638607No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC133490.11501551748797631TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG36250.019.72240446-47
GTATGCC39050.018.85546944-45
CGTATGC40300.017.79912244-45
GAGCACA93700.016.118919
AGAGCAC94200.016.083778
ACGTCTG50200.014.8540214-15
CTCGTAT49450.014.649528542-43
TCGTATG50050.014.61627842-43
ATCGGAA102300.014.11617852
ATGCCGT52450.014.03839946-47
AGTCACG52800.014.03419228-29
GCACACG53900.014.01053110-11
CGGGAAT32700.013.801281
TCGGAAG104750.013.6500283
CACACGT57600.012.78053112-13
ACACGTC58450.012.67592812-13
GTCACGA59900.012.29140828-29
CACGTCT63100.011.85493814-15
CCGGCAT24000.011.6784251
TGCCGTC66800.011.30787648-49
Produced by FastQC (version 0.11.2)