FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l04n01_lib_d8.351000000a20c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l04n01_lib_d8.351000000a20c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10553608
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT705110.6681222194343395No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400280.3792826112169412No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC130370.1235312132116334TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG35300.019.31109246-47
GTATGCC37900.018.61284444-45
CGTATGC38150.017.55699244-45
ATCGGAA78650.017.4551092
AGAGCAC82300.017.3705658
GAGCACA86550.016.9565989
TCGGAAG83350.016.2998623
GCACACG45200.016.28698210-11
ACGTCTG44900.016.02561214-15
CTCGTAT45600.015.10513442-43
CACACGT46850.015.00355812-13
ACACGTC47600.014.6673812-13
TCGTATG48300.014.55579742-43
ATGCCGT50500.014.15711546-47
GATCGGA102800.013.6786321
CGGAAGA102100.013.39954954
CACGTCT54200.013.05675714-15
CGGGCCT9450.012.5675961
CGGGCAT14400.012.5361791
TGCCGTC59350.012.24692648-49
Produced by FastQC (version 0.11.2)