FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l04n01_lib_d7.351000000a2043.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l04n01_lib_d7.351000000a2043.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10145187
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT451740.4452751831977074No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA201850.19896133999304302No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC175030.1725251589743984TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC87050.023.1876168
CGTATGC43550.021.4887144-45
TCGGAAG91900.021.192253
ATCGGAA93100.020.9190962
TATGCCG46050.020.47730646-47
GTATGCC47350.020.41628344-45
ACGTCTG49500.020.34103414-15
GAGCACA102950.019.8832289
CTCGTAT49750.019.14482742-43
TCGTATG50600.019.01098442-43
GCACACG53000.018.90786710-11
ATGCCGT55600.017.30183446-47
CGGAAGA116650.016.9401534
GATCGGA115850.016.8525331
CACGTCT64600.015.51287614-15
AGCACAC67650.015.12918210-11
CACACGT66900.015.014612-13
CGTCTGA67550.014.94094616-17
TGCCGTC66250.014.44970348-49
CAGTCAC69900.014.33714526-27
Produced by FastQC (version 0.11.2)