FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l04n01_lib_d6.351000000a1fcd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l04n01_lib_d6.351000000a1fcd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12334324
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT871220.7063378584833673No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510780.41411268262452No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC127710.10354033184145318TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC45550.015.5392944-45
TATGCCG44550.015.19505446-47
CGTATGC44450.015.12230544-45
AGAGCAC98500.014.7068428
GTCACGC47300.014.511250528-29
GCACACG49100.014.46157310-11
GAGCACA103650.013.8386429
ATCGGAA98700.013.7651342
TCGGAAG101500.013.5726153
ACGTCTG52400.013.55046914-15
CGCCAAT51550.013.45429832-33
CTCGTAT51000.013.31968442-43
AGTCACG52750.012.741837528-29
TCACGCC54300.012.59742830-31
TCGTATG59050.012.58990642-43
TATCTCG56900.012.18899138-39
ATGCCGT59750.011.84631946-47
GCCAATA60200.011.5211534-35
ACACGTC61300.011.4282212-13
CACACGT63250.011.41379512-13
Produced by FastQC (version 0.11.2)