FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l04n01_lib_d5.351000000a1f47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l04n01_lib_d5.351000000a1f47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17809912
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1361060.76421489336949No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA711090.39926643096271336No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC201540.11316170456092091TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG51150.021.26766246-47
GTATGCC58550.018.90380144-45
CGTATGC58200.018.69100644-45
ATCGGAA128000.017.7397632
GAGCACA138650.017.470089
AGAGCAC136650.017.2044228
TCGGAAG132500.017.137333
ACGTCTG73400.016.27384214-15
GCACACG74400.015.6399910-11
TCGTATG74400.014.97222742-43
ATGCCGT75750.014.95669546-47
CTCGTAT76750.014.73041842-43
ACACGTC79700.014.65928512-13
CACACGT81250.014.32117712-13
CGGAAGA169500.013.6205684
GATCGGA168150.013.6174541
CACGTCT88600.013.2943214-15
CACAGTG93600.012.12992332-33
AGCACAC103900.011.47364710-11
GTCACAC102650.011.1984928-29
Produced by FastQC (version 0.11.2)