FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l04n01_lib_d4.351000000a1ef5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l04n01_lib_d4.351000000a1ef5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10672273
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT968780.9077541400974282No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA516400.4838706805944713No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC125930.11799735632699801TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA74450.021.502592
TCGGAAG76150.020.6483673
AGAGCAC83500.019.5097568
TATGCCG36300.019.36818146-47
GAGCACA86750.018.888349
ACGTCTG44150.018.61017414-15
CGTATGC39350.017.98743444-45
ACACGTC47350.017.50279612-13
GTATGCC41650.017.1652144-45
GATCGGA95550.016.804511
GCACACG49350.016.64949810-11
CACACGT50000.016.43266912-13
CTCGTAT44550.016.20766442-43
CGGAAGA104650.015.3426724
CACGTCT55550.014.96197914-15
ACTGACC48150.014.79894532-33
ATGCCGT48300.014.75291646-47
TCGTATG49950.014.45548742-43
CGGGGAT26050.013.6770811
AGCACAC62100.013.26936210-11
Produced by FastQC (version 0.11.2)