FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l04n01_lib_d3.351000000a1e7e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l04n01_lib_d3.351000000a1e7e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11248625
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1371351.2191267821622644No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA737690.6558045983397971No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC145400.12926024291857893TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG36900.019.82595446-47
CGTATGC40550.018.09978944-45
ATCGGAA81700.017.9076842
AGAGCAC84950.017.834978
GAGCACA87350.017.453699
TCGGAAG84350.017.3451613
GTATGCC43200.017.09946444-45
ACTTAGG43950.016.69987332-33
ACGTCTG47250.016.23327614-15
TAGGCAT44400.016.15656934-35
TTAGGCA46050.015.8871634-35
TCGTATG48000.015.48831342-43
ATGCCGT50700.014.66378546-47
GCACACG52450.014.57889310-11
ACACGTC53800.014.3893712-13
CACACGT53050.014.18993212-13
CACTTAG51450.014.12617430-31
CTCGTAT56200.013.22845442-43
CTTAGGC56100.013.04071732-33
GATCGGA114150.012.9425071
Produced by FastQC (version 0.11.2)