FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l04n01_lib_d2.351000000a1e11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l04n01_lib_d2.351000000a1e11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3564319
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117560.3298245751853299No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC66830.18749724701969717TruSeq Adapter, Index 2 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56180.15761776653548687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14450.024.49281546-47
AGAGCAC34200.021.6634148
CGTATGC16700.021.05035444-45
GAGCACA37100.020.48219
GTATGCC18100.019.81584244-45
ACGTCTG19000.019.49625214-15
CGATGTA18950.018.67736434-35
TCGTATG19250.018.63190842-43
ATCGGAA39100.018.5875552
CTCGTAT19450.018.56244342-43
TCGGAAG39300.018.492963
ATGCCGT20300.017.7855746-47
CCGATGT20800.017.35876332-33
GCACACG21400.017.19951810-11
ACACGTC21200.017.13727612-13
CACACGT21850.017.06217612-13
TATCTCG20900.016.81978638-39
CACGTCT22950.016.03722214-15
CGGAAGA46950.015.6820814
ATGTATC23150.015.39023736-37
Produced by FastQC (version 0.11.2)