FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l04n01_lib_d12.351000000a2284.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l04n01_lib_d12.351000000a2284.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20736088
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT491200.23688171076434475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG60600.013.05200446-47
CGTATGC62600.012.78648744-45
GTATGCC64800.012.57230144-45
CGGGGAT56500.012.1071
CGGGCAT30150.011.8167071
CGAATTA45600.011.4584613
CGGAAAT52150.011.29509451
ATCGGAA140550.011.0175532
CGGGAAT57500.010.9876871
AGAGCAC152000.010.9674178
ACGTCTG76750.010.891355514-15
CGCGTAT19300.010.8297361
CTCGTAT77700.010.76003542-43
CGCGAAT41500.010.7597511
GCACACG76850.010.7534110-11
GAGCACA153050.010.4577289
CCGGCAT42700.010.3461221
CGGGCCT22950.010.1422871
CGCCCAT40950.09.9762441
ATGCCGT85250.09.91884846-47
Produced by FastQC (version 0.11.2)