FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l04n01_lib_d11.351000000a2234.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l04n01_lib_d11.351000000a2234.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11953363
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1467881.2280058758359467No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA616860.5160556071124084No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC327600.2740651312940132TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA110700.031.1959512
AGAGCAC117550.029.7776818
GAGCACA119050.029.6817559
TCGGAAG115000.029.6166823
TATGCCG60450.026.63914546-47
GTATGCC62850.025.81063544-45
ACGGCTA64300.025.00790632-33
CGTATGC64950.024.86640744-45
GATCGGA140200.024.5660861
CGGAAGA142050.024.3446454
GCACACG73200.024.10410710-11
ACGTCTG73000.023.90955214-15
CACACGT76750.022.89603412-13
CGGCTAC72550.022.52426532-33
ACACGTC78550.022.49229412-13
CTCGTAT72650.022.45958142-43
TCGTATG74400.021.96322342-43
ATGCCGT74650.021.92180646-47
AGTCACG75150.021.58505628-29
GCTACAT77700.021.12349134-35
Produced by FastQC (version 0.11.2)