FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l04n01_lib_d10.351000000a21bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l04n01_lib_d10.351000000a21bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7470909
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2018962.702428847681052No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA625410.8371270483953157No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC597240.7994207933733365TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC104300.060.1553768
GAGCACA111250.056.610829
ATCGGAA111250.056.1041372
TCGGAAG112100.055.3824843
GATCGGA125650.049.7917861
CGGAAGA125800.049.6906624
TATGCCG77500.038.98208646-47
CGTATGC78500.038.51511444-45
GTATGCC79050.038.24714344-45
GCACACG84150.037.6182910-11
ACGTCTG85250.037.10553414-15
GAAGAGC173400.036.3480156
TCGTATG84300.035.89277642-43
AAGAGCA177450.035.598497
ACACGTC89000.035.54126712-13
ATGCCGT85500.035.47353746-47
ACTAGCT87050.034.8143832-33
CACGTCT90950.034.75395214-15
CACACGT91750.034.6054112-13
TAGCTTA88300.034.21406634-35
Produced by FastQC (version 0.11.2)