FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l04n01_lib_d1.351000000a1dcf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l04n01_lib_d1.351000000a1dcf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8617183
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235620.27343042384036637No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA112440.1304834770249164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25350.015.927946-47
CGGGCAT13900.015.0378911
CGTATGC28150.014.42776244-45
GTATGCC30400.013.35991944-45
CGCAAAT20750.012.3630251
CGCGAAT15800.011.7261811
ATGCCGT34500.011.56585446-47
GAGCACA76650.011.4012229
CGGAAAT21000.011.3109671
TCGTATG35300.011.16894442-43
CGGGGAT21550.011.0222881
ACGTCTG39050.010.82473414-15
AGAGCAC78950.010.6479718
CTCGTAT38600.010.64480142-43
CCGGGAT21900.010.6292111
CGGGTAT18050.010.2644681
CGGGAAT29250.010.0696621
GCACACG41150.010.0414210-11
GGGAGAT74500.010.0113451
ACACGTC41950.09.90647812-13
Produced by FastQC (version 0.11.2)