FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l03n01_lib_c9.351000000a20f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l03n01_lib_c9.351000000a20f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6831623
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT423430.6198087921420723No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA169810.24856465293825492No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC109720.16060605217823057TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28550.020.05370546-47
CGTATGC29300.019.45930344-45
GTATGCC30100.019.02103844-45
TCGGAAG62200.018.8605733
AGAGCAC64100.018.8182628
ATCGGAA63450.018.4144252
GAGCACA68400.017.6352429
CTCGTAT35500.016.59583342-43
GCACACG36400.016.50420210-11
ATGCCGT34850.016.4966746-47
TCGTATG35350.016.3302442-43
CGGAAGA74700.015.767644
AGTCACG36500.015.74487728-29
ACGTCTG38850.015.15696414-15
CACACGT40500.014.89165612-13
ACACGTC40350.014.82932312-13
GATCGGA79300.014.7940821
GTCACGA39650.014.73359228-29
TGCCGTC41300.014.15019348-49
CACGTCT43300.013.92827614-15
Produced by FastQC (version 0.11.2)