FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l03n01_lib_c8.351000000a2086.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l03n01_lib_c8.351000000a2086.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14184392
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT785310.5536437515263256No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA377670.2662574469177107No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC207500.14628755324866938TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG55000.022.02799246-47
GTATGCC61000.020.21176344-45
CGTATGC61850.019.66513644-45
GAGCACA143200.018.3054929
AGAGCAC141150.018.100348
TCGTATG70550.017.54289442-43
ATGCCGT74800.016.6416846-47
ACGTCTG77750.016.33765214-15
ATCGGAA151700.016.3120842
TCGGAAG157400.015.8117273
CTCGTAT78850.015.57576742-43
GCACACG84700.015.16596210-11
CACACGT87450.014.58010412-13
ACACGTC88650.014.16847512-13
CACGTCT90900.013.94805314-15
CGGAAGA182000.013.856824
TGCCGTC91050.013.724174548-49
GTCACAC93400.013.39924628-29
TCTCGTA100150.012.75889340-41
GATCGGA194950.012.6934881
Produced by FastQC (version 0.11.2)