FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l03n01_lib_c7.351000000a201c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l03n01_lib_c7.351000000a201c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7107872
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT786041.1058724749123225No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA342790.48226811062438946No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC255350.359249575681723TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC81600.034.2768028
GAGCACA87750.031.9286129
ATCGGAA86000.031.7551962
TCGGAAG86050.031.7362983
TATGCCG45150.029.465546-47
CGTATGC47150.028.31640644-45
GATCGGA98450.027.8849451
GTATGCC48000.027.76547844-45
CGGAAGA99950.027.3694924
TCGTATG51750.025.75303342-43
ATGCCGT54450.024.82549346-47
ACGTCTG57450.024.34245114-15
CTCGTAT55900.024.18111642-43
GCACACG58600.023.74352510-11
ACACGTC58400.023.74335512-13
CACACGT59100.023.7031812-13
TGCCGTC60950.022.33381548-49
CACGTCT64300.021.7861314-15
CGTCTGA70500.019.87032716-17
TCTCGTA68100.019.77544440-41
Produced by FastQC (version 0.11.2)