FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l03n01_lib_c6.351000000a1f97.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l03n01_lib_c6.351000000a1f97.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7628950
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT507850.6656879387071615No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA239350.3137391121976157No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC136400.17879262545959798TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC70800.020.5248648
TATGCCG33050.020.48655746-47
GTATGCC34700.019.71780844-45
GAGCACA75200.019.387099
CGTATGC35250.019.20796444-45
ATCGGAA74850.018.845392
GTCACGC36900.018.21260328-29
TCGGAAG78250.018.1477033
ACGTCTG40250.017.6378114-15
ATGCCGT40300.017.33156846-47
TCACGCC39750.017.02610830-31
TCGTATG40600.016.96916442-43
CGCCAAT40500.016.88660232-33
CACGCCA41500.016.2509230-31
GCACACG45950.015.65738310-11
AGTCACG43600.015.63174228-29
ACACGTC47050.015.3413912-13
CGGAAGA93550.015.3315684
CACACGT46500.015.318612-13
CTCGTAT45700.015.23140242-43
Produced by FastQC (version 0.11.2)