FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l03n01_lib_c5.351000000a1edb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l03n01_lib_c5.351000000a1edb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16598953
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT667800.40231453152497026No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA387610.2335147283084662No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC187350.11286856466187958TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG54400.018.95242346-47
CGTATGC58150.017.73018544-45
GTATGCC60600.016.81736444-45
GAGCACA141300.015.66179
AGAGCAC142550.015.25785358
CTCGTAT70700.014.85156342-43
ATGCCGT74150.014.09663546-47
ATCGGAA151600.013.8789292
ACGTCTG78500.013.85308914-15
TCGTATG76700.013.72074542-43
TCGGAAG157300.013.3759663
GCACACG83850.013.08294110-11
ACACGTC87400.012.52416512-13
CACACGT86950.012.50705912-13
CACGTCT88150.012.28268514-15
CGGGGAT43650.011.53406051
CACAGTG93050.011.43315532-33
CGGAAGA191400.011.3153464
TGCCGTC97650.011.21523848-49
GTCACAC100150.011.02580628-29
Produced by FastQC (version 0.11.2)