FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l03n01_lib_c4.351000000a1e55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l03n01_lib_c4.351000000a1e55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10410053
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT672690.6461926754839769No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA345680.3320636311842024No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC125330.12039323911223122TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37000.017.59159346-47
GTATGCC39550.016.57745644-45
AGAGCAC87100.016.5748188
CGTATGC39550.016.2771444-45
GAGCACA89950.016.0496569
TCGGAAG92450.015.3091353
ATCGGAA92250.015.1366072
ACGTCTG47500.014.99581714-15
GCACACG49050.014.619493510-11
ACTGACC46800.014.360432-33
TCGTATG47350.013.94677742-43
ACACGTC51650.013.83732712-13
CACACGT51850.013.6465712-13
CTCGTAT50050.013.62156142-43
ATGCCGT50250.013.189383546-47
CGGGGAT27050.012.9938271
CACGTCT57100.012.64095814-15
CCGGCAT20050.012.5555031
GATCGGA116050.012.0330251
CGGAAGA118100.011.983994
Produced by FastQC (version 0.11.2)