FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l03n01_lib_c3.351000000a1df6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l03n01_lib_c3.351000000a1df6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6046010
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT507670.8396777378800233No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA241920.40013165707631976No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC102430.16941751667628732TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG24350.021.07298946-47
TCGGAAG51850.020.5190333
CGTATGC25500.020.49528144-45
ATCGGAA53150.020.0173232
ACTTAGG26500.019.8939232-33
GAGCACA57000.019.8289039
GTATGCC27450.019.29895844-45
AGAGCAC57450.019.2602738
GCACACG29700.018.70794310-11
CACTTAG28800.018.05795130-31
TAGGCAT29450.017.9813434-35
TTAGGCA30450.017.3908234-35
GATCGGA62900.017.1415791
ACGTCTG32350.017.1016314-15
ATGCCGT31600.016.76444446-47
CTCGTAT31750.016.75971642-43
TCGTATG31300.016.62119142-43
CGGAAGA64900.016.6122344
ACACGTC34850.016.01128812-13
CTTAGGC33150.015.97477132-33
Produced by FastQC (version 0.11.2)