FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l03n01_lib_c2.351000000a1d99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l03n01_lib_c2.351000000a1d99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9170207
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163910.17874187572865038No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC123930.13514416850132172TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33900.018.42988246-47
GTATGCC36900.016.99589744-45
CGTATGC36750.016.67742244-45
AGAGCAC85250.015.76518
GAGCACA88650.015.4283129
TCGTATG41650.014.94323242-43
CGATGTA43800.014.3131934-35
ACGTCTG48000.014.19714914-15
ATGCCGT45500.013.99232746-47
TCGGAAG93700.013.888673
ACACGTC49150.013.67202512-13
ATCGGAA95650.013.5558682
CCGATGT47200.013.53389632-33
TATCTCG47600.013.47071638-39
CTCGTAT47250.013.27273242-43
GCACACG50550.013.24661710-11
CACACGT52900.013.06192712-13
CACGTCT54300.012.28759714-15
GATGTAT54050.012.12606934-35
CGGGGAT26700.011.384741
Produced by FastQC (version 0.11.2)