FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l03n01_lib_c12.351000000a2241.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l03n01_lib_c12.351000000a2241.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18268614
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAT45600.010.1031461
GTCGAAT60050.09.0956681
CGCAAAT55300.09.0180761
GGAAGAT267200.09.0120021
CTCGGAT94900.09.0085741
GGGAAAT93000.08.8861991
CCCGCCT52100.08.8426771
GGGAGAT147400.08.7321571
CTCGGTT116550.08.7206981
CTGAAAT104700.08.7097221
CGGGGAT56450.08.5819491
GGGGAAT57150.08.4768341
GTCAAAT81950.08.4616231
CTGGGAT87350.08.4278841
CGATAAT45900.08.3815061
CTCGAAT80550.08.2549081
CGGAAAT52050.08.2124271
GGCAGAT90300.08.09995651
CGCTAAT29350.08.0911761
CGGGCAT35300.08.0728391