FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l03n01_lib_c11.351000000a21c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l03n01_lib_c11.351000000a21c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11089658
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT609550.5496562653239622No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA143370.1292826162898802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG35200.015.18536946-47
AGAGCAC80800.014.5754428
ACGGCTA38700.014.54278332-33
GAGCACA84800.014.0559199
GCACACG43400.013.51327410-11
GTATGCC41500.013.22355844-45
CGTATGC41500.013.16631444-45
ACGTCTG43800.013.11810514-15
ATCGGAA87800.012.4412392
TCGGAAG90900.012.1214463
ACACGTC49450.011.81167212-13
TCGTATG48150.011.5450642-43
ATGCCGT49100.011.41868246-47
CGGCTAC49400.011.34475732-33
GCTACAT51600.011.13694334-35
AGTCACG53000.010.79807828-29
CACACGT53650.010.709969512-13
CACGGCT53250.010.65840830-31
GGCTACA55700.010.57296634-35
CCGGCAT21450.010.4067371
Produced by FastQC (version 0.11.2)