FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l03n01_lib_c10.351000000a2178.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l03n01_lib_c10.351000000a2178.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13260472
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1767231.3327052008405131No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA827420.6239747725420332No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC181380.13678246143877834TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG45550.021.6966946-47
GTATGCC49550.019.60957544-45
CGTATGC52350.018.65149744-45
GAGCACA117650.018.2038129
AGAGCAC117450.018.1943788
TAGCTTA57400.017.45808634-35
ATCGGAA120750.017.1487622
TCGGAAG121850.016.9549123
CTCGTAT60150.016.62749542-43
ACGTCTG64100.016.14971214-15
GCACACG68950.015.668467510-11
ATGCCGT64550.015.49438846-47
TCGTATG64650.015.47012942-43
ACACGTC69000.015.14074512-13
CACACGT71250.014.92920912-13
GATCGGA146350.014.2143271
CGGAAGA147950.014.05991654
TATCTCG72500.014.05213738-39
ACTAGCT72300.013.991837532-33
CACGTCT78200.013.57178814-15
Produced by FastQC (version 0.11.2)