FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l03n01_lib_c1.351000000a1d62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l03n01_lib_c1.351000000a1d62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11527329
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT742400.6440347109031069No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA361130.3132815936805482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33050.014.59232846-47
GTATGCC36950.013.05210844-45
CGGGGAT28150.012.8222031
CGTATGC37650.012.49393744-45
CCCGCAT17000.012.2922441
GAGCACA85050.012.2833669
AGAGCAC86150.012.1816478
ACGTCTG44250.011.6966514-15
CGCGTAT11100.011.1244311
ACACGTC46450.010.83626812-13
ATGCCGT44950.010.78202946-47
GCACACG47950.010.74511510-11
TCGGAAG95050.010.392723
CGGGAAT32450.010.3913291
CTCGTAT46300.010.36462142-43
TCGTATG46750.010.3156742-43
ATCGGAA94500.010.3024392
CACACGT48800.010.21713412-13
CCCATAA40450.010.2135197
CCGGGAT32150.09.8974031
Produced by FastQC (version 0.11.2)