FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l02n01_lib_b9.351000000a1fe7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l02n01_lib_b9.351000000a1fe7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6864496
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410750.5983687658933737No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC185720.2705515452263356TruSeq Adapter, Index 9 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA134110.1953675841605851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA70550.030.021099
AGAGCAC72000.029.1526748
ATCGGAA71650.028.7679942
TCGGAAG72850.028.424513
TATGCCG36100.026.9780746-47
CGTATGC37350.026.07471544-45
GTATGCC38600.025.16878744-45
GATCGGA85300.024.2777421
CGGAAGA88750.023.7065454
CTCGTAT41850.023.66742742-43
AGTCACG40950.023.66150728-29
ACGTCTG45600.023.01543614-15
TCGTATG43100.022.92590742-43
GCACACG45900.022.91657310-11
ATGCCGT43250.022.46318846-47
CACACGT48000.021.71602212-13
ACACGTC49500.021.48967412-13
GTCACGA46750.020.92915528-29
CACGTCT55450.019.14113814-15
TGCCGTC51000.019.04980548-49
Produced by FastQC (version 0.11.2)