FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l02n01_lib_b8.351000000a1f54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l02n01_lib_b8.351000000a1f54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10612458
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350170.3299612587394928No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA158840.14967314829420292No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC147390.13888394187284417TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG39250.020.3353746-47
GTATGCC41800.019.43542944-45
CGTATGC43350.018.52131744-45
GAGCACA93250.017.9261059
AGAGCAC95100.017.8270658
CTCGTAT51550.016.03474642-43
ACGTCTG52350.015.96581714-15
ATCGGAA100350.015.9027152
TCGGAAG103050.015.6243193
ACACGTC55650.015.23228812-13
TCGTATG54900.015.09957142-43
ATGCCGT55150.014.81717446-47
GCACACG56800.014.63126410-11
CACACGT59500.014.04713712-13
CACGTCT64300.013.03553714-15
GATCGGA125800.012.9124051
CGGAAGA132000.012.6653784
GTCACAC69950.011.91653728-29
CGTCTGA73200.011.71072616-17
TGCCGTC70900.011.69334548-49
Produced by FastQC (version 0.11.2)