FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l02n01_lib_b7.351000000a1f3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l02n01_lib_b7.351000000a1f3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12247948
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT438980.3584110579176202No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA228850.1868476254144776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG36150.013.40457546-47
AGAGCAC82800.012.9051668
CGTATGC38750.012.566215544-45
CGGGGAT38700.012.3963181
GTATGCC41050.011.97786844-45
CTCGTAT44700.011.84943142-43
CGGAAAT31450.011.7802971
ACGTCTG45600.011.61231214-15
GCACACG45650.011.59966610-11
TCGTATG44400.011.07356742-43
GAGCACA101800.010.8230979
ATGCCGT49250.010.75548146-47
CGCCCAT23600.010.6670941
ATCGGAA93700.010.6447492
TCGGAAG94850.010.6158363
CGGGCAT22900.010.163491
CACACGT53350.010.10346112-13
CCCGCAT16250.09.9382021
CGGCAAT36450.09.5127841
CCGGCAT24650.09.2492511
Produced by FastQC (version 0.11.2)