FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l02n01_lib_b6.351000000a1eb1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l02n01_lib_b6.351000000a1eb1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10051451
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT732510.7287604545851142No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA366680.36480305181809075No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC193160.19217125965196466TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA97150.022.2412439
AGAGCAC96650.022.1597638
TATGCCG45050.022.04093246-47
TCGGAAG99150.021.268293
ATCGGAA98850.021.2367422
CGTATGC48700.020.38843344-45
GTATGCC50300.019.97601944-45
ACGTCTG54800.019.62809214-15
GTCACGC51300.019.5814128-29
CTCGTAT53300.019.16224942-43
AGTCACG54850.018.57383528-29
TCACGCC55400.018.26261330-31
GCACACG60300.017.95593310-11
CGGAAGA118350.017.938314
TCGTATG57150.017.74666842-43
GATCGGA120950.017.5539251
ACACGTC61650.017.40863612-13
CGCCAAT58600.017.02322232-33
ATGCCGT58450.016.90663746-47
CACGTCT64100.016.89146414-15
Produced by FastQC (version 0.11.2)