FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l02n01_lib_b5.351000000a1e48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l02n01_lib_b5.351000000a1e48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14937625
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1185630.79372055463971No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA612600.41010535476690574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG46400.014.28308646-47
CGTATGC48550.013.60157644-45
GTATGCC50550.013.29838744-45
GAGCACA108750.013.1879429
CTCGTAT54100.012.77639542-43
CGGGCAT23150.012.7210051
AGAGCAC114800.012.5343018
CGCGAAT29500.011.9149021
ATCGGAA117100.011.7219112
ACGTCTG59150.011.72188514-15
TCGGAAG116300.011.6392263
GCACACG61600.011.60255510-11
ACACGTC60150.011.52693112-13
GGCGAAT41550.011.0887211
CACACGT61700.011.0834212-13
ATGCCGT60200.011.04834946-47
TCGTATG62150.010.93043642-43
CGTTCTA42050.010.61931234-35
CGGGAAT42500.010.5055721
GCCAGAT58050.010.2279491
Produced by FastQC (version 0.11.2)