FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l02n01_lib_b4.351000000a1de9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l02n01_lib_b4.351000000a1de9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14204174
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321190.2261236732245043No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC252660.17787729156232526TruSeq Adapter, Index 4 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA151070.10635606125354422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG62150.020.98227146-47
GAGCACA138150.020.2817579
CGTATGC65200.020.07342344-45
AGAGCAC139150.019.931238
GTATGCC66000.019.83010744-45
ATCGGAA149150.017.928152
ATGCCGT75800.017.70518746-47
TCGTATG75550.017.57429742-43
TCGGAAG153900.017.4982573
ACGTCTG79650.017.23111714-15
CTCGTAT76600.017.17835642-43
ACTGACC76800.017.00925432-33
ACACGTC86900.015.87556812-13
GCACACG87100.015.86643210-11
CGGAAGA183400.015.0721084
CACACGT92350.014.91296612-13
GATCGGA185250.014.5119881
CACGTCT97200.014.2176614-15
TGCCGTC94200.014.19681748-49
CGTCTGA106600.013.09837816-17
Produced by FastQC (version 0.11.2)