FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l02n01_lib_b3.351000000a1d8c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l02n01_lib_b3.351000000a1d8c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9104028
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT566300.6220323575454734No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA297870.3271848460923011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG24600.013.5185446-47
GTATGCC28300.012.338346544-45
CGTATGC28900.011.99999644-45
GAGCACA61950.011.651829
CGGGGAT23550.011.4963851
CGCGAAT18950.011.0286111
AGAGCAC70200.010.95896158
ACGTCTG32300.010.87985314-15
CGTTCTA23650.010.84680934-35
ATCGGAA59250.010.82154752
ACTTAGG31650.010.80632232-33
CCCGCAT12500.010.6396021
CGGGAAT23000.010.5322141
TTAGGCA35450.010.38543934-35
CTCGTAT33900.010.29967442-43
TCGGAAG63050.010.1693933
AAAAACC85700.010.1417812
CGCCCAT15050.010.099291
ACACGTC35800.010.08131712-13
CGGCAAT22200.010.055931
Produced by FastQC (version 0.11.2)