FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l02n01_lib_b2.351000000a1d3c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l02n01_lib_b2.351000000a1d3c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8490729
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211770.2494132129290665No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86740.10215848368261429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA57250.013.3551059
TATGCCG26200.012.78387546-47
CCGGCAT17500.012.48440551
GTATGCC29150.012.22319144-45
CGTATGC27350.012.15913444-45
GCACACG30650.012.08540110-11
ATCGGAA56550.012.00962
CGGGGAT24150.011.9966721
AGAGCAC63100.011.665398
CGCGTAT8601.8189894E-1211.597621
TCGGAAG59000.011.5913913
CGGGAAT25900.011.5528431
ACGTCTG31450.011.24915114-15
ACACGTC31800.011.20017112-13
CGGGCAT15350.011.1388971
CGGCAAT23750.010.7988681
CACACGT35200.010.320703512-13
TCGTATG33550.010.2654842-43
CGATGTA33550.010.12397834-35
GGGGAAT27050.010.0081931
Produced by FastQC (version 0.11.2)