FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l02n01_lib_b12.351000000a2142.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l02n01_lib_b12.351000000a2142.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31562672
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870950.9096029639062244No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA951880.30158409909021644No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC554570.1757043890327156TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC263000.023.7089168
ATCGGAA257000.023.5998922
TATGCCG122300.023.4628546-47
GAGCACA277200.022.7171069
TCGGAAG271850.022.2758263
CGTATGC130500.022.0972844-45
GTATGCC137350.021.15087944-45
GCACACG152600.020.6796510-11
ACGTCTG156600.019.86316714-15
TCGTATG151000.019.25345242-43
ATGCCGT157600.018.7049246-47
CGGAAGA336400.018.2837834
CACACGT170900.018.25672712-13
GATCGGA339650.017.941681
ACACGTC178200.017.46886312-13
CTCGTAT168600.017.24360342-43
GTCACCT173950.016.62868328-29
CACGTCT191650.016.34198814-15
TGCCGTC193700.015.37872248-49
AGCACAC207900.015.28179510-11
Produced by FastQC (version 0.11.2)