FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l02n01_lib_b11.351000000a20bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l02n01_lib_b11.351000000a20bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10196276
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530340.5201310753063177No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA273300.26803903699742926No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC159430.15636100866630132TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC84400.023.1256878
GAGCACA88400.022.1329989
TATGCCG39300.021.94071846-47
ATCGGAA91400.020.5262722
CGTATGC42350.020.41631144-45
ACGGCTA42800.019.92244732-33
GTATGCC45100.019.32941844-45
TCGGAAG97000.019.2922843
GCACACG51800.018.83984610-11
ACGTCTG51000.018.76272414-15
ACACGTC54050.017.96758512-13
CTCGTAT50800.017.06629842-43
TCGTATG51250.016.91644542-43
CACACGT57700.016.83098612-13
ATGCCGT52850.016.80983246-47
CGGCTAC53150.016.57916632-33
GCTACAT52900.016.4787734-35
AGTCACG52800.016.28209328-29
CGGAAGA119200.015.8187954
GATCGGA119750.015.6675831
Produced by FastQC (version 0.11.2)