FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l02n01_lib_b10.351000000a2036.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l02n01_lib_b10.351000000a2036.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9388635
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730080.7776210279769105No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA369520.39358224065585673No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC106160.11307288013646287TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC65350.018.4568218
TATGCCG29300.017.75577746-47
GTATGCC32050.016.97306444-45
CGTATGC32650.016.15186144-45
TCGGAAG71300.015.8528413
ACGTCTG37650.015.7657214-15
ATCGGAA71150.015.7527642
GCACACG37050.015.63653210-11
GAGCACA84200.014.7196419
TCGTATG37100.014.597712542-43
TAGCTTA36950.014.33569934-35
CTCGTAT39000.013.58201142-43
ACACGTC44400.013.26188312-13
ATGCCGT42000.013.17861746-47
CACACGT45900.013.08712712-13
GCGGAAT17050.012.2568261
TATCTCG44700.012.2223838-39
CACGTCT49800.011.91926414-15
GTCACTA46250.011.65582828-29
CGGAAGA98450.011.5775114
Produced by FastQC (version 0.11.2)