FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l02n01_lib_b1.351000000a1d05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l02n01_lib_b1.351000000a1d05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13650518
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286480.20986749367313387No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC182640.13379712037301442TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG48700.018.92531646-47
CGTATGC51450.018.14430444-45
GTATGCC53200.017.32420344-45
CTCGTAT57300.016.4152842-43
ATGCCGT62150.015.02075646-47
TCGTATG66250.014.12596242-43
GAGCACA140250.014.0181929
AGAGCAC144200.013.5353998
ACGTCTG73300.012.92510914-15
TGCCGTC79100.012.25308648-49
CACACGT82150.011.61933912-13
ACACGTC81700.011.56708612-13
TCTCGTA82550.011.53805440-41
CACGTCT83800.011.39061914-15
CGTTCTA40300.011.37518234-35
GTCACAT84650.011.36197628-29
CATCACG87950.011.20719432-33
TCACGAT84650.011.19572234-35
ATCACGA92900.011.14748534-35
GCACACG86650.011.09816110-11
Produced by FastQC (version 0.11.2)