FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l01n01_lib_a9.351000000a21e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l01n01_lib_a9.351000000a21e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7363459
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590290.8016477038848182No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC223310.30326779846265184TruSeq Adapter, Index 9 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA153420.2083531666299765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC76900.031.8665358
ATCGGAA75150.031.8558882
GAGCACA77800.031.253739
TCGGAAG77000.031.0288313
TATGCCG41250.028.3337546-47
GATCGGA87250.027.4393541
CGGAAGA89850.026.695494
CGTATGC44550.026.39402644-45
GTATGCC46100.025.76423544-45
ACGTCTG48450.025.24032614-15
GCACACG48300.025.07289910-11
AGTCACG47600.024.89667928-29
TCGTATG48100.024.78953742-43
GTCACGA48700.024.28556428-29
ATGCCGT49700.024.09001246-47
CTCGTAT49700.023.80031642-43
ACACGTC54300.022.1710712-13
CACGTCT54900.022.0586714-15
CACACGT56500.021.43385512-13
TGCCGTC56400.021.31256948-49
Produced by FastQC (version 0.11.2)