FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l01n01_lib_a8.351000000a215f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l01n01_lib_a8.351000000a215f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6688048
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1286651.9238049726915836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA852771.2750656095769648No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC113880.17027389755575917TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25300.023.47330546-47
CGTATGC25450.023.42759144-45
AGAGCAC53100.023.0742768
GTATGCC27650.021.56355544-45
TCGGAAG58850.020.1787283
GCACACG30700.019.80042310-11
ATCGGAA60150.019.6636452
GAGCACA63600.019.4888529
ACGTCTG30400.019.44913514-15
TCGTATG30950.019.41656342-43
CTCGTAT31500.019.22835442-43
ATGCCGT32650.018.77116646-47
CACACGT35000.017.4356412-13
CACGTCT36700.016.69275914-15
CGGAAGA71550.016.5301574
TGCCGTC39700.015.55765348-49
ACACGTC39650.015.21119812-13
GATCGGA79300.015.0957131
AGCACAC42500.014.63811810-11
CAGTCAC42400.014.33812726-27
Produced by FastQC (version 0.11.2)