FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l01n01_lib_a7.351000000a20d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l01n01_lib_a7.351000000a20d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10521244
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177590.16879182727821918No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC155680.147967293601403TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC42150.018.59714944-45
TATGCCG45750.017.13384846-47
GTATGCC46200.016.8640544-45
CTCGTAT51850.015.20914742-43
ATGCCGT52850.014.74215346-47
TCGTATG53000.014.65505442-43
AGAGCAC123650.013.3273768
GAGCACA128250.013.2566229
TGCCGTC62500.012.38995348-49
ACGTCTG66150.012.38404114-15
CGGGCAT17550.012.1800181
TCTCGTA73900.010.89604240-41
GCACACG75500.010.69318710-11
TCCAGTC77550.010.5335224-25
CAGTCAC76750.010.36537426-27
CACGTCT79950.010.33555814-15
CGGGAAT34250.010.263221
CGTCTGA82350.010.23705516-17
AGTCACC80900.010.09863128-29
CCAGTCA80150.010.01455626-27
Produced by FastQC (version 0.11.2)