FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l01n01_lib_a6.351000000a206c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l01n01_lib_a6.351000000a206c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15979940
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT519860.3253203704144071No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA249070.15586416469648823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCTA54350.011.581202534-35
CGGGGAT44500.011.5289451
CGGGAAT47600.011.0775021
CCCATAA64750.011.0754078
CGGCAAT54000.010.116491
CGGGCAT29350.010.0348161
GCCAGAT67450.09.64860251
ACCCATA73900.09.6399196
AAAAAAC224800.09.4246311
CGCCCAT32950.09.2267841
CGGAAAT40450.09.1601341
GGAAAAT108800.08.9942321
CGCGGGT19550.08.9904371
CGGCATT43950.08.97108651
CCCTGCC84050.08.90073470-71
TATGCCG39000.08.83118346-47
GCGAATT35550.08.818862
CGCGTAT15100.08.80861
GGGAGAT141550.08.5576811
GGGGAAT46100.08.3466051
Produced by FastQC (version 0.11.2)