FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l01n01_lib_a5.351000000a1fda.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l01n01_lib_a5.351000000a1fda.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10901541
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT476960.4375161273071394No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA234610.21520810681719219No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC212060.19452295780935924TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG49950.022.8275546-47
GAGCACA108500.021.8846979
GTATGCC51800.021.8744544-45
CGTATGC52650.021.74689344-45
TCGGAAG112200.020.7859823
AGAGCAC116200.020.3936378
ATCGGAA117550.019.839872
CTCGTAT60000.019.35839342-43
TCGTATG61150.018.76127642-43
ATGCCGT62150.018.49940746-47
ACGTCTG64650.018.40083114-15
GCACACG68450.017.41410-11
ACACGTC69250.017.28136412-13
CGGAAGA135450.017.252584
GATCGGA137050.017.0869981
CACACGT72350.016.3111712-13
CACGTCT73400.016.23962214-15
CACAGTG74250.015.38719132-33
TGCCGTC77200.014.89304948-49
CGGGGAT32300.014.8530871
Produced by FastQC (version 0.11.2)