FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l01n01_lib_a4.351000000a1f60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l01n01_lib_a4.351000000a1f60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11874148
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT911840.7679203594228402No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA418940.3528168926309492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA78000.015.2856572
GAGCACA88900.014.4775739
TATGCCG37500.014.37877146-47
AGAGCAC89800.014.3325358
TCGGAAG85450.014.064153
GTATGCC39400.014.04710744-45
CGTATGC41250.013.24436344-45
GCACACG48300.013.17607110-11
ACGTCTG47600.013.17003714-15
CGGGCAT21950.012.3356951
GATCGGA103200.011.6916911
ACACGTC53650.011.59643112-13
TCGTATG48350.011.3973342-43
ATGCCGT51000.011.13153246-47
CACGTCT57200.011.04270814-15
CTCGTAT49250.010.99613942-43
ACTGACC50350.010.99168932-33
CGGAAGA111550.010.7732084
CCCGACT17250.010.7398651
CCCATAA47000.010.7111998
Produced by FastQC (version 0.11.2)