FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l01n01_lib_a3.351000000a1ece.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l01n01_lib_a3.351000000a1ece.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9093993
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316370.34788898561940834No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA152470.16766012465591296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27400.015.17202146-47
GAGCACA67650.014.1098759
CGGGGAT24200.013.740551
GTATGCC31400.013.69308644-45
ACGTCTG33000.013.59910614-15
CGTATGC31600.013.23055344-45
CGTTCTA25800.012.79762934-35
ACTTAGG33100.012.70215332-33
AGAGCAC72100.012.6463628
ATCGGAA68450.012.629912
TCGGAAG69000.012.4603963
CACTTAG36300.012.17080430-31
CTCGTAT33400.012.16114842-43
TAGGCAT35000.011.94480634-35
TCGTATG38650.011.61548142-43
GCACACG39700.011.60315610-11
TTAGGCA37250.011.47838334-35
CACACGT39650.011.43806812-13
CCCATAA38200.011.4373168
ACACGTC40050.011.26454412-13
Produced by FastQC (version 0.11.2)