FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l01n01_lib_a2.351000000a1e3b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l01n01_lib_a2.351000000a1e3b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15120839
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT575210.3804087855177878No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA231460.15307351662166366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG42250.014.6746146-47
ATCGGAA97650.013.0853022
GAGCACA106250.012.9173549
TCGGAAG99600.012.7813813
GTATGCC49450.012.72981944-45
GCACACG54150.012.62899110-11
CACACGT53400.012.49507912-13
CGATGTA48900.012.43522634-35
CGTATGC50250.012.43261244-45
ACACGTC55700.011.85123312-13
ACGTCTG55100.011.85107814-15
CTCGTAT55050.011.77937242-43
AGAGCAC111450.011.71810158
CCGGGAT36300.011.3849021
CGTTCTA47200.011.17206734-35
GATCGGA119900.010.8950741
CACGTCT63100.010.80013814-15
CGGGGAT39500.010.7030991
ATGCCGT60900.010.68774346-47
CCCATAA59000.010.4639588
Produced by FastQC (version 0.11.2)