FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l01n01_lib_a12.351000000a235d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l01n01_lib_a12.351000000a235d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24693522
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC78900.012.13289544-45
TATGCCG80350.012.00282846-47
GTATGCC82150.011.71072644-45
CGGGAAT62350.011.1997441
CGGAAAT69350.010.4802641
CGGCTAT64150.010.4411821
CGGGGAT81350.010.2773791
ATGCCGT98900.09.96770746-47
CTCGTAT98150.09.84939942-43
TCGTATG99400.09.749433542-43
ATTACGC61000.09.576724-25
CTCGGAT121550.09.4577461
GGGAGAT197900.09.4095211
CGCAAAT64100.09.3376961
CGGGCAT41450.09.1683821
GGGGAAT75950.09.1317151
AAAAAGT221500.09.0927942
TATTACG61350.08.86403724-25
GGGAAAT135950.08.8403311
CGCCAAT58050.08.83789351
Produced by FastQC (version 0.11.2)