FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l01n01_lib_a11.351000000a22e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l01n01_lib_a11.351000000a22e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10306351
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1036561.0057487853848563No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA448850.43550816384964963No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC158790.15407004865252502TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA84300.020.5628249
AGAGCAC83850.020.56018
TATGCCG38900.020.51760746-47
ATCGGAA82750.020.2640042
TCGGAAG85000.019.7835883
CGTATGC40500.019.4135444-45
ACGGCTA42550.019.14724532-33
GTATGCC43450.018.25948144-45
ACGTCTG46450.018.25038714-15
GCACACG48550.017.41189810-11
CTCGTAT47600.016.61670342-43
ACACGTC51350.016.55495312-13
CACACGT51500.016.23008312-13
GATCGGA104350.016.2065621
TCGTATG49150.016.1893342-43
AGTCACG49150.016.04224828-29
CGGAAGA108950.015.6518934
ATGCCGT52250.015.41169746-47
GCTACAT52000.015.25661134-35
CACGTCT57900.014.60027314-15
Produced by FastQC (version 0.11.2)