FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l01n01_lib_a10.351000000a226a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l01n01_lib_a10.351000000a226a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9423350
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2313282.454838247544663No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA958001.0166235998875135No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC400990.42552807653329233TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC112500.036.051498
TCGGAAG113950.034.975743
ATCGGAA114100.034.9297562
GAGCACA120500.033.7760859
CGTATGC64750.029.67841144-45
TATGCCG65800.029.3852446-47
GTATGCC66550.028.94707544-45
GATCGGA139400.028.7282661
CGGAAGA140100.028.5819994
TCGTATG70450.027.2763842-43
GCACACG74800.026.92029410-11
CTCGTAT72000.026.6561942-43
TAGCTTA71250.026.53567534-35
ACGTCTG75150.026.44741214-15
ATGCCGT75000.025.68563746-47
TATCTCG74650.025.6782438-39
CACTAGC74950.025.57355930-31
ACTAGCT75150.025.47470532-33
TCACTAG78750.024.67129730-31
TGCCGTC82450.023.7105248-49
Produced by FastQC (version 0.11.2)